- Akio Ootsuka (Ghost in the Shell, Uchu Sentai Kyuranger) as Master Alude, the Cosmo Beast Grand Master
- Kenjiro Tsuda (Yu-Gi-Oh!, Kamen Rider Revice) as Instructor Phoros, the Wielder of Thunder, Master of Lightning White Tiger Fist
- Toshiyuki Morikawa (JoJo's Bizarre Adventure, Zyuden Sentai Kyoryuger) as Tubahn, the Wielder of Fire, Master of Flaming Red Dragon Fist
- Miku Ito (The Quintessential Quintuplets, Kamen Rider Revice) as Spica, the Wielder of Ice, Master of Rapid Frozen Panther Fist
- Toshiki Masuda (Samurai Flamenco, My Hero Academia) as Albeo, the Wielder of Wind, Master of Leaping Spirit Crane Fist
- Tomoaki Maeno (Cells at Work!, ULTRAMAN) as Pharood, the Wielder of Water, Master of Phantom Water Snake Fist
First making his debut in Ultra Galaxy Fight: The Destined Crossroad, this spin-off series will reveal his origins. At the start of the series, he ends up on the Planet D60, close-to-death. His only memory is his name, Regulos. He is scouted by Master Alude who takes him into the Cosmo Beast Style martial art, and trains alongside his fellow disciples.
The team behind the previous three Ultra Galaxy Fight series namely, director Koichi Sakamoto (Ultraman Geed, Kamen Rider Fourze) and writer Junichiro Ashiki (Ultra Zero Fight) returns to work on this spin-off series. Ultraman Regulos is set to premiere soon on Tsuburaya Imagination in Japan, and in Ultraman Connection worldwide.