- Roger Craig Smith (Resident Evil) as Batman
- Tony Hale (Arrested Development) as the Joker
- Grey Griffin (Avatar: The Last Airbender) as Catwoman
- Tara Strong (Teen Titans) as Harley Quinn & Poison Ivy
- Fred Tatasciore (Family Guy) as Gorilla Grodd & Deathstroke
- Yuri Lowenthal (Ben 10: Omniverse) as Robin
- Adam Croasdell (Reign) as Nightwing & Alfred
- Will Friedle (Boy Meets World, Batman Beyond) as Red Robin & Red Hood
- Tom Kenny (SpongeBob SquarePants) as Penguin
- Eric Bauza (The Adventures of Puss in Boots) as Two-Face
This project, which is three years in the making is directed by Junpei Mizusaki (JoJo's Bizarre Adventure anime opening sequence) and written by Kazuki Nakashima (Gurren Lagann, Kamen Rider Fourze) and character designs by Afro Samurai creator Takashi Okazaki. The film will be animated by Kamikaze Douga (JoJo's Bizarre Adventure anime opening sequence) and music by Yuugo Kanno (JoJo's Bizare Adventure: Diamond is Unbreakable).
Batman Ninja will be available digitally on April 24th and will be available on home video on May 8th.