- Hayato Sano (Kaguya-sama: Love Is War) as Aoi Mibuomi
- Nogizaka46's Erika Ikuta (Psychic Power Research Club's 3 People) as Sakura Miharutaki
- Shiori Akita (Aku no Hana) as Tsuzura Hanatemari
- Minori Hagiwara (As the Gods Will) as Yukimi Togakushi
- Mijika Nagai (Lust In A Karaoke Box) as Sachiko Juraku
- Hinatazaka46's Mirei Sasaki (Re:Mind) as Mikura Sado
- Riko Fukumoto (Keep Your Hands Off Eizouken!) as Kokoro Aiura
- Atsuhiro Inukai (Kamen Rider Build) as Nagi Kamishimo
Kakegurui Twins takes place a year prior to the events of the main Kakegurui series as it follows Mary Saotome and how she got her start at this gambling-addicted school.
The director of the live-action Kakegurui film, Tsutome Hanabusa will serve as the show's chief director as well as write the series, while Shinya Nagano, who direct the previous two seasons of the live-action Kakegurui series will direct the spin-off. Live-action Kakegurui writer Minato Takano will return as well.
Kakegurui Twins is set to premiere on Amazon Prime on March 26th, and will run for eight episodes, with two episodes premiering each week.
[Source: Anime News Network]