Ai Fairouz (How Heavy Are the Dumbbells You Lift?) plays Jolyne Cujoh in the series, also part of the cast are: Mutsumi Tamura (Digimon Adventure tri.) as Ermes Costello, Mariya Ise (Hunter x Hunter 2011) as Foo Fighters, Atsumi Tanezaki (Dragon Quest: The Adventure of Dai) as Emporio Alnino, Yuichiro Umehara (Goblin Slayer) as Weather Report, and Daisuke Namikawa (Fate/Zero, Engine Sentai Go-Onger) as Narciso Anasui. Daisuke Ono also returns to reprise his role as Jotaro Kujo.
David Production returns to animate the series with Kenichi Suzuki from Parts 1-3 returning as the chief director, and Part 4's Toshiyuki Kato as the director. Yasuko Kobayashi will once again handle series composition, Masanori Shino (Black Lagoon) as character designer, Part 5's Shun'ichi Ishimoto as Stand designer, Yoshikazu Iwanami also returns as sound director, and Parts 3-5's Yugo Kanno returns to compose the show's music.